This MATLAB package is used to extract, visualize, and quantify spatiotemporal membrane signaling of time resolved 3D confocal microscopy observed single cells. It provides
- basic cell parameter, such as cell volume and membrane area
- 3D animation of extracted membrane signaling
- Mollweide projected 2D animation of extracted membrane signaling
- detrended fluctuation analysis (DFA) for each membrane site, quantification and 2D Mollweide projected map of DFA parameter
- preparation for WAVOS (Morlet Wavelet) analysis
- an example dataset of a Dictyostelium cell
CITING: Please cite the following papers when using this code:
1) Hörning, M. and Shibata, T. (2019). Three-Dimensional Cell Geometry Controls Excitable Membrane Signaling in Dictyostelium Cells. Biophysical Journal 116, 372–382. https://doi.org/10.1016/j.bpj.2018.12.012
2) Hörning, M., Bullmann, T. and Shibata, T. (2021). Local membrane curvature pins and guides excitable membrane waves in chemotactic and macropinocytic cells - biomedical insights from an innovative simple model. Frontiers in Cell and Developmental Biology, 9, 2467. https://doi.org/10.3389/fcell.2021.670943
The following open sources have been used:
Mesh Generator:
Persson, P.-o. and Strang, G. (2004). A Simple Mesh Generator in MATLAB. SIAM Review 46, 2004
Download: http://persson.berkeley.edu/distmesh/
Detrended Fluctuation Analysis:
Abrar Habib (2017). r-DFA : Robust Detrended Fluctuation Analysis
https://www.mathworks.com/matlabcentral/fileexchange/60026-r-dfa-robust-detrended-fluctuation-analysis, MATLAB Central File Exchange
引用格式
Marcel Hörning (2024). 4D Cell Membrane Visualization and Quantification (https://www.mathworks.com/matlabcentral/fileexchange/97802-4d-cell-membrane-visualization-and-quantification), MATLAB Central File Exchange. 检索来源 .
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R2021a
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1.0.12 | changes in the description |
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1.0.11 | improved description |
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1.0.10 | changed image |
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1.0.9 | change image |
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1.0.8 | updated image |
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1.0.7 | file update |
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1.0.6 | modified description |
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1.0.5 | modified description |
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1.0.4 | modified description |
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1.0.3 | modified installation path |
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1.0.2 | modified description |
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1.0.1 | description modifications |
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1.0.0 |