How can I find branchpoints this retina image?

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Hi, I found vessel image from retinal image color. But my project must find all vessel lengths and one how much tortuosity have one main vessel?Mathemetical if I find brancpoints of my image,I can bring ut vessel length with spline. What do you think about this problem?

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Image Analyst
Image Analyst 2013-12-3
If you skeletonize with bwmorph(), then you can find the branchpoints, also with bwmorph().
I don't know what "I can bring ut vessel length with spline" means.
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Image Analyst
Image Analyst 2013-12-3
It expects the "foreground" to be white, so invert your image before doing further morphology, such as skeletonization.

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