It really depends upon what you want. If you only want to track a single point, or a few points within your image series this can be done very easily with the image processing toolbox function impixel:
The following script will let you open up a series of JPEG files, and allow you to interactively identify single or multiple points of interest in each image. The image coordinates are output to a cell matching the order of the input images, coords.
% Open all images using dialogue box
[FileName,PathName] = uigetfile('.JPG', 'select image files','multiselect', 'on');
images = transpose(FileName);
% open each image and interactively define the point of interest using impixel
% store output in a cell (coords)
coords = cell(size(images,1),1);
for i = 1:size(images)
im = imread(strcat(PathName, images{i}));
[xi,yi,P] = impixel(im);
coords{i} = horzcat(xi,yi); % store xy coordinates from each point of interest
end
You may need to be more specific if you want something more sophisticated (i.e. to track all non white pixels.
Thomas
