How to vectorize this loop? Subsequental subtraction of columns within a matrix

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I'm trying to vectorize the following loop:
a=rand(1000,100);
j=1;
k=2;
tic
for i=1:50;
b(:,i)=a(:,j)-a(:,k);
j=j+1;
k=k+1'
end
toc
Is there any easier way to subsequently subtract an entire column from the previous column within a matrix?
Thanks, Charles

采纳的回答

the cyclist
the cyclist 2014-6-3
b = a(:,1:end-1) - a(:,2:end);

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