I understand that you wish to obtain your playlist in a MATLAB App Designer app.
If you have the collection of audio files in a particular location, you can use functions like 'audioread' and 'audioplayer' by providing the file location to read and play the audio respectively.
You can refer to the following links to understand better how your desired result could be achieved through App Designer:
You can refer to the following MathWorks documentations to know more about these functions:
Hope this helps! Thanks.