The name field of the struct returned from dir() contains only the basic filename without any directory information. If you are working with a different directory than the current directory, then you need to put in the directory name. We recommend using fullfile() for that.
Example:
project = 'Scalp7';
Dir = dir(fullfile(project, '*.png'));
filename = Dir(1).name;
fullname = fullfile(project, filename) ;
img = imread(fullname) ;