I understand that you are using the Live Data Acquisition (1.3) with a NI6001 data acquisition board, MATLAB® R2021a, Data Acquisition Toolbox and want to use two channel acquisition (voltage) at same time.
To perform two-channel data acquisition simultaneously, you can use the ‘addinput’ function to add multiple input channels to the acquisition session.
For further information, refer to the documentation link below:
