Hi Meghraj Kc,
As per my understanding you want to know how to create Region of Interest (RoI) mask for a large multiband image as you are not able to read it.
It is possible to read such a large multiband image by using "multibandread" function in MATLAB. Please refer to the example below for reading a multiband image.
filename = 'multibandImage.tif'; % Replace with the actual filename and path
image = multibandread(filename, [height, width, 2500], 'uint16', 0, 'bsq');
Please refer to the below documentation link to learn more about "multibandread" function.
Please refer to the following MATLAB Answer which solves a similar problem.
There are a number of ways to create RoI masks for an image depending upon the shapes, color-range, and use-cases.
Please refer to the following documentation link to learn about different functions for "RoI-Based Processing".
Hope it helps!
