Take the number of columns of the matrix "freq", take the log to the basis 2 of this number, take the nearest positive integer (>= this number), take (2^this number) and multiply the result by 2.
E.g. if freq has 64 columns, you get 64 = 2^6 -> 6 -> 6 -> 2^6 = 64 -> 2*64=128 = Nfft.