Sarah, try this nice framework: http://www.mathworks.com/matlabcentral/fileexchange/24224-magic-matlab-generic-imaging-component
Description:
This GUI will help the novice user get up to speed very quickly on using GUI-based applications. Everything is laid out in a very simple Step 1, Step 2, Step 3, etc. layout. It is a very good starting point for a typical image analysis application. This application uses GUIDE to do the user interface design, and has most of the basic controls such as buttons, listboxes, checkboxes, radio buttons, scrollbars, etc. It allows the user to select a folder of images, select one or more images and display them, to select a series of options, and to individually or batch process one or more images. The user can ...............
Alternatively, try writing your own from scratch after watching this tutorial: http://blogs.mathworks.com/videos/category/gui-or-guide/