n_files = 10;
VACF_all = zeros(n_files,100); % Pre-allocate a matrix to store all the VACF results.
% I assume the VACF you calculate for each file is a 1x100 vector (no idea if that's true)
for ii = 1:n_files
file_name = sprintf('exp%d.dat',ii);
% ...
% load the file, calculate VACF
% ...
VACF_all(ii,:) = VACF; % store VACF as the ii-th row of VACF_all
end
mean_VACF = mean(VACF_all, 1); % take the mean of all 10 VACFs