deconvoluting z stack multichannel tif image (3D volumetric image)

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Chanille
Chanille 2023-3-17
编辑： Chanille 2023-3-17
Hello, I have been having trouble deconvoluting my image in matlab:
% Load multi-channel color Z stack image
img = imread('ZStack1.tif');
% Define the parameters for the deconvolution algorithm
numIterations = 20; % Number of iterations
PSF = fspecial('gaussian', 7, 1); % Point spread function
background = 'none'; % Background handling method
noiseModel = 'poisson'; % Noise model
% Preallocate deconvolved image
deconvolvedImg = zeros(size(img), class(img));
% Deconvolve each color channel separately
for i = 1:size(img, 3)
% Extract the ith color channel
channel = img(:,:,i);
% Deconvolve the channel
% = deconvlucy(channel, PSF, numIterations, [], background, noiseModel, 'edge', 'same');
deconvolvedChannel = deconvlucy(channel, PSF, numIterations, [], background, noiseModel, 'edge', 'same');
% Store the deconvolved channel in the output image
deconvolvedImg(:,:,i) = deconvolvedChannel;
end
% Display the original and deconvolved images side by side
figure;
montage({img, deconvolvedImg}, 'Size', [1 2]);
title('Original (left) and deconvolved (right) image');
When it works it does not convolute, I think the issue is mainly that it doesn't read into matlab as a multidimensional multichannel (z stacked image) any suggestions to correct this? Thanks!

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