How to perform image segmentation

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Chathu
Chathu 2015-4-29
评论： Chathu 2015-5-13
I want to manually segment a small object(eg: a feature similar to cell nucleus) which is located within the original image. Can anyone kindly tell me what is the cause to obtain the boundary of the segmented image larger than the real image? (though i drew the boundary along the cell nucleus, segmented image still gives larger segmentation.
Thank you.

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采纳的回答

Image Analyst
Image Analyst 2015-4-29
  7 个评论
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Image Analyst
Image Analyst 2015-5-12
Chathu, that's what my Image Segmentation Tutorial does. http://www.mathworks.com/matlabcentral/fileexchange/25157-image-segmentation-tutorial Just tell it to get the dark things instead of the bright things. It will crop them out into separate subimages. Then you can just save the subimages with imwrite().
Chathu
Chathu 2015-5-13
Image Analyst- thank you so much for the tutorial and your responses. Really admire your effort.

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更多回答（2 个）

Thomas Koelen
Thomas Koelen 2015-4-29
If I understand you correctly, you want to extract only the nucleus, and not the background, this is impssible because you can't make an image that's not a rectangle. You can however make the backround white.
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Chathu
Chathu 2015-5-12
Thomas: please excuse me for replying you late. But yes i understand your point. I am still trying to figure it out.

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Chathu
Chathu 2015-5-12
@ Image Analyst and Thomas- i was trying hard to segment this (pls see the attachment- arrow indicates the portion that i need to segment). Can anyone of you kindly tell me what is the best way/method to segment this?
  2 个评论
Thomas Koelen
Thomas Koelen 2015-5-12
Since the part you are trying to get is way darker than the other parts I suggest you can do something like this:
(im using a paint image because you didn't supply your own image wihout the arrow)
IM=imread('chathu.png');
figure
IM(IM>30)=255;
bw1=im2bw(IM);
imshow(bw1);
IM2=medfilt2(bw1);
stats = regionprops(IM2,'Centroid');
which give soyu the center of the "blob":
stats =
Centroid: [134.3417 148.9362]
Chathu
Chathu 2015-5-12
@ Image Analyst,
@ Thomas,
Thank you so much for both of your responses. Really appreciate it. Please kindly note that i need to segment(extract) the black speck in a separate window. Since i have huge set of black specs like this, my intention is to segment all black specs, save them in a separate folder, then stack them and finally, model it.

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