Why is my function not defining my outputs and just returning "ans"?

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I have written the following function to remove noise from an EMG signal

function[EMG_filtered] = noise_removal(EMG)
Fs = 2000;
[b1,a1]=butter(5,[48/Fs*2, 52/Fs*2],'stop');
[b2,a2]=butter(5,300/Fs*2,'low');
[b3,a3]=butter(5,10/Fs*2,'high');
x = filter(b1,a1,EMG);
x1 = filter(b2,a2,x);
EMG_filtered = filter(b3,a3,x1);

The function is running correctly except for the fact that it does not save the output as EMG_filtered. It returns "ans" which equals EMG_filtered but this is overwritten as soon as a different function is run. I am having this problem with all the functions I am running. Am I not defining the output correctly?

Thanks for any help and advice