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I create a matlab GUI using 2 push buttons and 2 axes.I got some error. The error is

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Error using axes Invalid object handle
Error in astgui>pushbutton3_Callback (line 329) axes(handles.axes2);imshow(B)
Error in gui_mainfcn (line 96) feval(varargin{:});
Error in astgui (line 42) gui_mainfcn(gui_State, varargin{:});
Error in @(hObject,eventdata)astgui('pushbutton3_Callback',hObject,eventdata,guidata(hObject))
Error while evaluating uicontrol Callback

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