How to do standardization for the brightness and contrast of the image?

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Tan Chee Siang
Tan Chee Siang 2016-8-1
% Image Loading
a= imread('D01-1.jpg')
figure
imshow(a)
%Area definition
user_defined_ellipse = imellipse(gca, [10 10 275 275]);
wait(user_defined_ellipse);
MASK = double(user_defined_ellipse.createMask());
%mask
mask = uint8(MASK);
I = a.*mask;
figure
imshow(I)
title('comparison')
% Binary Image
Inew=im2double(I);
maxValue=max(Inew(:));
Inew(Inew == 0) = inf;
minValue = min(Inew(:));
Ibefore=im2double(I);
INew= imadjust(Ibefore, [minValue maxValue], [0 1]);
figure
imshow(INew)
title('adjusted')
INew2=im2uint8(INew)
figure
imshow(INew2)
title('adjusted2')
%Calculation
Bw1=I>0;
figure
imshow(Bw1)
title('Bond Area')
thresholdvalue=150;
Bw2=I>thresholdvalue;
figure
imshow(Bw2)
title('Intensity of IMC')
Bondarea = length(find(Bw1 == 1));
IMCArea = length(find(Bw2 == 1));
areaRatio = IMCArea/Bondarea*100;
fprintf('The area ratio is %0.2f%%\n', areaRatio) % %
As u can see here, i am using imadjust to adjust all my image from[min max] to [0 1] Then i use colour thresholding by selecting the value as 140 . Unfortunaly it does not work. Any way to adjust all the image to same contrast and brightness?

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