Alex - if you can assume that when you launch your GUI that all elements in the table are empty, then you can make the decision whether you need to move the first and second columns to the fourth and fifth respectively. Try
function pushbutton1_Callback(hObject, eventdata, handles)
% get the table data
tableData = get(handles.uitable1,'Data');
% if all elements in the first column are not empty then...
if all(~isempty(cell2mat(tableData(:,1))))
% move columns one and two to columns four and five
tableData(:,4) = tableData(:,1);
tableData(:,5) = tableData(:,2);
end
% update columns one and two with your data
tableData(:,1) = mat2cell(randi(255,6,1),ones(6,1));
tableData(:,2) = mat2cell(randi(255,6,1),ones(6,1));
set(handles.uitable1,'Data',tableData);
The use of randi is just an example. You would copy your data from elsewhere in order to populate these first two columns.
