How do image segmentation in SegmentTool?

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Dear all,
I have image, which I would like to segmenting in SegmentTool. I imported this image into this application and marked interesting area for segmentation and confirmed segmentation by "enter" to saving in workspace. But when I want to export and save results, it appears "No circles detected" (below the window). How do I make those circles? I attach printscreen.
Thank you for your answers.

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Image Analyst
Image Analyst 2016-9-6
You could use imfreehand() first to draw a black line at the top and bottom of the "circle" to separate it from the tissue at the top and bottom.

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