Calculate mean with same ROI on multiple images

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Ihiertje 2017-2-1

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https://ww2.mathworks.cn/matlabcentral/answers/322855-calculate-mean-with-same-roi-on-multiple-images

评论： Adam 2017-2-2

I used the function mmROI to draw three ROIs and calculate the mean value of this ROI in multiple images. Next, I corrected the orginal images and now I want to calculate the mean value again, using the same ROIs. However, I cannot get it done. I tried to use poly2mask and roipoly, but haven't found the solution yet. Below are the two things I've tried.

if true
  I = Fluo_Corrected_ExpTime(:,:,:,1);
J = imadjust(I);
BW = roipoly(X1, Y1,J,720,1080);
imshow(J)
hold on
plot(X1, Y1, 'r.-', 'MarkerSize',15);
end
if true
  I = Fluo_Corrected_ExpTime(:,:,:,2);
J = imadjust(I);
imagesc(I)
ROI1 = poly2mask (X1, Y1,720,1080); %mxn is size image
Bimg = imagesc(ROI1)
end

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Ihiertje 2017-2-1

@Adam: the first time I used the function mmROI in which the mean value is calculated. However, I want to use the ROI defined in this function to use in other images. Therefore, I cannot do it the same way.