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Extracting white blood cell from cell samples

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Mona Madhukar
Mona Madhukar 2011-3-15
评论： Navin Kumar 2020-5-22
Hi,
I am working on leukemia images. http://www.clinicalflow.com/Cases/Case_List/Acute_Lymphoblastic_Leukemia_%28ALL%29_general.
I need to extract only the purple cells, which are also the largest cells as opposed to others. Plz let me know how to extract only those cells , and also how i can find the area, mean and intensity of these cells alone. Kindly help..
Thank you,
Mona

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回答（1 个）

Sarah Wait Zaranek
Sarah Wait Zaranek 2011-3-16
This should be enough to get you started. I filtered the purple cells by looking at the blue color plane. I then removed any noise - and used region properties to get the area of the cells. You can use region properties to get a list of pixels and use that to calculate mean values from the original image.
%%Reading in the image
myImage = imread('L1+blast.jpg');
figure
imshow(myImage)
%%Extracting the blue plane
bPlane = myImage(:,:,3) - 0.5*(myImage(:,:,1)) - 0.5*(myImage(:,:,2));
figure
imshow(bPlane)
%%Extract out purple cells
figure
BW = bPlane > 29;
imshow(BW)
%%Remove noise 100 pixels or less
BW2 = bwareaopen(BW, 100);
imshow(BW2)
%%Calculate area of regions
cellStats = regionprops(BW2, 'all');
cellAreas = [cellStats(:).Area];
%%Superimpose onto original image
figure, imshow(myImage), hold on
himage = imshow(BW2);
set(himage, 'AlphaData', 0.5);
  4 个评论
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murk hassan memon
murk hassan memon 2017-2-9
i am facing this type of error when i am converting gray from original and then extracting blue pan kindly help me to resolve this problem and if i am making any mistake then also tell me the write way to code
Navin Kumar
Navin Kumar 2020-5-22
Hi,how can i segment cells and nucleus separately?By applying the above code,only the nucleus get segmented.So i applied lab color space and kmeans.For some images only i got correct answer,please let me know how to extract cells.

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