hema - you will want to call your function (m-file) from the pushbutton callback. If you are using GUIDE to create your GUI, see GUIDE push button callback. For example,
function pushbutton1_Callback(hObject, eventdata, handles)
% call your function
myFunction(...); % which may or may not take any input parameters
I'm assuming that your m-files are in fact functions and not scripts.
App Designer Callbacks may also prove useful if you are using App Designer.
