"ecgpuwave" class WFDB not work on my .mat ECG file

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MM Asim
MM Asim 2017-8-3
i am trying to detect "P" wave with the help of "ecgpuwave" class using "Waveform-Database-Toolkit" in MATLAB-2016a but End-of-File (EOF) error occur every time...tell me how to solve it plz

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