Your plot function should be factored out from the callback and simply take the inputs it needs to update the plot. This depends on what is being plotted. Ideally if it is e.g. a single line plot that updates you should plot it once, for the first time, then keep its handle, then future updates should just change the XData and YData (and any other properties that may need updating) of this line object.
Either way though you should just do something like this:
function plot( someArgs )
% do plotting update
function pushButtonCallback( ... )
% Get relevant arguments
plot( ... )
function checkboxCallback( ... )
% Get relevant arguments
plot( ... )
function radioButtonCallback( ... )
% Get relevant arguments
plot( ... )
