Detecting centroid and orientation of capsules
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I have a stack of 128 binary images like this one:
I want to detect centroid and orientation of capsules in 3D but, as you can see, many capsules have regions connected to other objects that I want to exclude from the detection procedure. I tried to use regionprops3, but I am not sure if this is the right MATLAB function to be used in this case. I think that, if I am able to impose the detection of my shape, I could get the expected results. Do you know if there is a method suitable for this task?
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Anton Semechko
2018-5-29
If your capsules have parametric a representation (e.g., ellipsoid), you can use Hough transform (HG). Description of a HG variant designed specifically for detecting ellipsoids can be found here: Partitioned Hough transform for ellipsoid detection
Alternatively, if you are able to segment-out individual objects from your image, you can compute for each object a set of features invariant to rigid motion (e.g., aspect-ratio, surface area, volume, etc.). You can then use these features to discriminate between capsules and other objects present in your data.
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Anton Semechko
2018-6-4
To get started, I suggest you look into nonlinear filters (e.g., nonlocal means, diffusion, bilateral, median, etc.) so you can remove as much noise from the data as possible prior to segmentation.
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