Is it possible to use imwrite function with standalone gui?

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I have created a GUI which writes the result as an image in a directory using imwrite. My program is working good in Matlab compiler. Further, I have created a standalone application using Application compiler of Matlab for that GUI. BUt the standalone GUI is not responding whenever I am using the imwrite function with the given directory.
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Hossain Md Shakhawat
编辑:Hossain Md Shakhawat 2018-8-27
In details: I have created a gui that will upload an image (user will select the image). Then I have written the uploaded image to a folder called Input. Further, I have read the image from the input folder and performed some image processing using a separate Matlab program. The GUI then calls the program and display the output image produced by that program. Finally, the GUI also writes the output into the Output folder. I tried several procedures including the above one but can't write the input image(I guess). As the program cant read the image from the input folder. I have converted the GUI to standalone using the Matlab Application compiler. And tested the results using the test only files. **The GUI and program work fine in Matlab compiler (not standalone).

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