Image substraction in multiple frames

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How to substract previous frame between next frame in multiple frames with in a loop? Provide codes.

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KSSV
KSSV 2018-9-18
I1 = rand(50) ; % say a initial frame
for i = 1:N
I2 = rand(50) ; % say a present frame
I1 = I2-I1 ;
end

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