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I have two images taken by projecting laser light onto a agar plate. We have taken images at starting point and after an interval of 20 minutes, where we cultured bacteria on it. Then we applied imsubtract(a,b), (b,a) both giving different answers ?

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GUDIVADA ROKESH KUMAR
关闭： MATLAB Answer Bot 2021-8-20
a=imread('im5.jpg');
b=imread('im7.jpg');
c=imsubtract(a,b):
imshow(c)
d=imsubtract(b,a);
imshow(d)
after that in the output side we got greenish and red color image. Here we used a red color laser light. My question is, is that greenish color represents bacterial colony or anything else. Why the output is changing after interchanging these a,b to b,a in 'imsubtract' function? Following are the images: mat1 - for imsubtract(a,b) and mat2 - for imsubtract(b,a).

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KALYAN ACHARJYA
KALYAN ACHARJYA 2018-12-20
编辑：KALYAN ACHARJYA 2018-12-20
Yes, how you expect that both gives the same results?
A-B !=B-A (!=>Not Equal)
Note: Subtraction and division functions are Not Commutative.
In your case
imsubtract(a,b)=a-b
and
imsubtract(b,a)=b-a
Both are not same.
  2 个评论
GUDIVADA ROKESH KUMAR
GUDIVADA ROKESH KUMAR 2018-12-20
Thank you for your response. But I attached the output images, from that could you tell me what resembles these green colored area in it.
KALYAN ACHARJYA
KALYAN ACHARJYA 2018-12-20
编辑：madhan ravi 2018-12-20
I have answered, what you have asked in the question. I hope you also agreed that both subtraction results are not same.
Though I will try- Define the following

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