substraction two images at a roi

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Anastasia 2019-2-12

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https://ww2.mathworks.cn/matlabcentral/answers/444544-substraction-two-images-at-a-roi

关闭： MATLAB Answer Bot 2021-8-20

Hello,

I have two videos (40x40 pixels each with around 300 images each) from fluorescence imaging with some bleed through from one channel (channel 2 in the image) to the other (channel 1 in the image) only in the center of the image (picture attached). I tried substracting, but the problem is that the channel causing the bleed through has a lot of background noise, so it also substracts pixels at other region. Is it possible to do a program with matlab where I would give him the coordinates at which the substraction is to be done?

Thank you very much in advance.