Detection of blob in a sperm cell

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cliffy
cliffy 2019-5-26
编辑： cliffy 2019-5-27
I was trying to detect how many vacuoles are there in a sperm cell. Following the picture.
I have thought of a few ways, first is perform the laplace of gaussian to the image, it will give me the gradient of the map, and I may get the boundary. But there is one problem, the boundary is not closed, so it will be hard to tell how many blobs are there.
I wonder if anyone have any different ideas.

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