Using FLIRT Registration Transformation Matrix in MATLAB

Question

Sara4mat 2019-9-11

1
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此问题的直接链接

https://ww2.mathworks.cn/matlabcentral/answers/479946-using-flirt-registration-transformation-matrix-in-matlab

Hello,

I have registered a 3D image (moving) to another 3D image (reference) using FLIRT. Both of these images are anisotropic with same spacing (voxel size). After registration, FLIRT saves the 4D transformation matrix as a .mat file. When I load it in MATLAB, it looks like this:

[a,b,c,d;
 e,f,g,h;
 i,j,k,l;
 0,0,0,1]

Now, I want to use this transformation matrix in MATLAB, but first I want to make sure that I can load it properly in MATLAB and its coordinate system because the FSL/FLIRT is using a coordinate system in mm not voxel coordinate system. For this purpose, I am applying the same transformation matrix to the moving image in MATLAB to make sure that the MATLAB registered image is the same as the FLRIT registration output. Therefore, I run the following code in MATLAB:

T = load('FLIRTtransformation'); % FLIRT output transformation 
T = T'; 
T(4,1:3)=T(4,1:3)./spacing; % FSL/FLIRT works in mm coordinate space not voxel coordinates 
tform = affine3d(T); 
R = imref3d(N); % N is image size 
img_transformedMATLAB = imwarp(img,tform,'OutputView',R); 

However the registered image from MATLAB and FLIRT are different. I tried some other modifications of T matrix, but none of them gave me the same registration result.

I would appreciate any help in this regard.