if your data is called 1.mat, 2.mat, 3.mat and so on, and in addition each of these data contains a structure called "Data" and in this same structure there is a field called "Data" and in this field There is a matrix of which you want the last column, so the solution is as follows:
matrix=[];
for k=1:50
dd=load(num2str(k,'%i.mat'));
matrix=[matrix dd.Data.Data(:,end)];
end
disp(matrix)
Espero te sea de ayuda y sea la solución de tu requerimiento
