Tiff import into a matrix becomes integer only

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Joe Sheppard
Joe Sheppard 2019-12-11
Hello all,
I've modified some code I found online which can quickly import tiff files into matrices in MatLab. Speed is important for me because I have 20 stacks of 1200x1200x250.
The code imports the tiffs just fine, but it is changing the actual pixel values of the data.
Before import the tifs are 8-bit (0-255) but all pixels values lay between 0-5 (with decimal places).
When I important into matlab all the pixel values become whole integers which means I lose all the data since my range is so small.
I have tried changing the data type from uint8, uint16, single and double but this does not solve the issue. I have attached my code below:
files = dir ('*.tif'); % identify all tif files in the current directory
for j=1:size(files,1) % this loop extracts info about the tifs, and saves it to a structure array called "files"
FileTif= files(j).name;
InfoImage=imfinfo(FileTif);
mImage = InfoImage(1).Width;
nImage = InfoImage(1).Height;
NumberImages = length(InfoImage);
FinalImage=zeros(nImage,mImage,NumberImages,'single');
TifLink = Tiff(FileTif, 'r'); % it also makes a tiff library which I don't understand - but the internet said this would load them into matlab faster
for i=1:NumberImages
TifLink.setDirectory(i);
FinalImage(:,:,i)=TifLink.read();% each tiff is read into matlab as an individual 3D matrix - just like a tiff stack
end
FinalImage = single(FinalImage);
TifLink.close();
files(j).data = FinalImage; % the matrix is then written into the "files" structure array
end

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