Based on your code, no zero padding is done and no window function is applied. To do this, you can give fft a second input for fft length. If it is larger than the actual length of the signal, zero padding will be done automatically. for example:
FTSignal = fft(Signal-meanSignal, 10240)/N;
You can apply a window function by this
FTSignal = fft((Signal(:)-meanSignal) .* hann(length(Signal)) ) / N;
By this, you will apply a hanning window.
There isn't anything that you must do honestly. But in general it is oftentimes true that by applying a window function before fft, you will better handle spectral leakage.
Applying zero padding helps with frequency resolution--> to have the spectral trace at the expected frequency. Your frequency resolution is equal to Fs/fftLength. But this doesn't help with differentiating two close frequency trace. To get better differetiation capability, you need longer signal.
