Correlate pixel color to load value

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I'm working on a tensile sensor based image and I would like to create an equation to correlate pixel color to load. How can I do it without image processing toolbox? I have RGB colored image and the actual load at which that image was captured.
In the image below, the effective load is 500N.
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Himanshu Verma
Himanshu Verma 2020-9-24
@Image Analyst, it's sort of an industrial project and they need to use the tool in future and they don't have the Image Processing Toolbox license. That's why looking for alternative.
@Walter Roberson, yes, the total measured load would double but the individual profiles would stay the same.
Walter Roberson
Walter Roberson 2020-9-24
Yup, I would definitely recommend calibration with known forces. And area of an object such as a penny should be easy enough to calculate especially as it is already known https://www.bluebulbprojects.com/measureofthings/results.php?p=1&comp=area&unit=in2&amt=0.442&sort=pr.

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