What is the amplitude unit when we plot wav. files?

Hello, I work with bioacoustics and in my analysis I usually use softwares like Raven 1.4 and Adobe Audition. However, I've been urged to apply Matlab in my analysis.
I need to calculate underwater sound pressure levels. I usually take the values provided by Adobe (in dBFS) and perform the necessary conversions and calculations to obtain SPL. In Matlab I need to what is the amplitude unit when I open wav. files.
Can I calculate SPL directly from the wav. file using the amplitude levels and calibration data or do I need to insert a calibration file separately?
Thank you, Lis

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Thank you for reading my question. I understand that the amplitude range is –1.0 <= y <= +1.0. What I would like to know is if the values contained in this range are voltage values or if they are a normalized plot of voltage values that must be converted before the SPL calculation.
Lis
They are normalized, with -1 to correspond to the minimum voltage that can be produced by the line driver, and +1 to correspond to the maximum voltage that can be produced by the line driver.

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Wav files come in two varieties. The first has a range -1 to 1 and the second has the range 0 to 255. For the first 20*log10(x) corresponds to dB FS while for the second 20*log10((x-128)/255) gives dB FS.

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You forgot to divide the x by P0= 20e-6
he is right you are wrong. he said its dB FS in that case you dont need to divide but if its dBSPL then you have to divide by P0

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