Hi,
You can import your dataset from an Excel sheet into MATLAB using the "Import Data" button at the top of your MATLAB workbench. If your data is always going to be in the same format, then you can also generate a script/function while importing the data so that you don't have to manually import the data every time. Also based on your data you can import it as individual column vectors, a single matrix, a cell array or a table.
Hope this helps.