Automated Counting of Bacterial Colonies

版本 1.0.0.0 (175.1 KB) 作者: Daniel Asarnow
A function to automate bacterial colony counting in images of plated agar in a Petri dish.
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更新时间 2012/4/2

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Provides colony_count.m to automate counting of bacterial colonies.

The approach is as follows:

1. Use Hough transform to locate Petri dish.
2. Threshold with Otsu's method.
3. Count colonies as regional maxima in the original image which are present in the segmentation.

David Young's circular Hough transform functions are required. They are available here:
http://www.mathworks.com/matlabcentral/fileexchange/26978-hough-transform-for-circles

Currently, a range of Petri dish radii is required as a parameter.

This program was originally developed for a Stack Overflow question found here: http://stackoverflow.com/questions/9954891/counting-colonies-on-a-petri-dish.

Future plans are to increase versatility by automating parameter selection and reducing sensitivity to experimental and imaging conditions (lighting, bright/dark field, media color). Features suggested in comments will be considered for addition.

引用格式

Daniel Asarnow (2024). Automated Counting of Bacterial Colonies (https://www.mathworks.com/matlabcentral/fileexchange/35943-automated-counting-of-bacterial-colonies), MATLAB Central File Exchange. 检索时间: .

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致谢

参考作品: Hough transform for circles

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1.0.0.0