This method first estimates a cutoff, then calculates the distribution of pixels within the cutoff in order to determine the final threshold.
I have found this method to work quite well for fluorescence microscopy images in which most of the pixels are background and the background is approximately gaussian.
There are a couple parameters than can be used to tune the threshold.
引用格式
Jake Hughey (2026). Simple Image Thresholding (https://ww2.mathworks.cn/matlabcentral/fileexchange/44291-simple-image-thresholding), MATLAB Central File Exchange. 检索时间: .
