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how can i use image which is save in variable?

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in the following code i wanted to use gray image which saved in variable b for further use,but it gives an error "undefined b" on second line... kindly sort this out
CODE:
i=imread('C:\Users\Romesha\Desktop\eye.jpg');b=rgb2gray(i);imshow(b); im = imread(b); imshow(im);

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