How to calculate sampling intensity along perpendicular line?

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Saranya
Saranya 2014-2-5
Hi all... Can anyone help me to calculate sampling intensity along perpendicular line. this is my input image http://postimg.org/image/krhtig9cd/098b6a85/ this was the output which i get till now.. http://postimg.org/image/s1y63ktv9/e53cefc7/

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