I exactly want to do same thing explained in "what should I do so that only gray image converts into binary , not the black backgroung while moving the threshold." question. But when I count white and black pixels in ROI I am getting 0.

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Kirti 2014-2-21

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评论： Renuka 2014-2-23

I exactly want to do same thing explained in "what should I do so that only gray image converts into binary , not the black background while moving the threshold." question. But when I count white and black pixels in ROI I am getting 0.

Following is my code.

if true
   close all;
   clear all;
   %Read image in Matlab
   ReadImg = imread('Sanjivjadhv25rckd.jpg');
   figure(1); imshow(ReadImg);title('Original Image');
   %Convert image into Gray Image
   GrayImg = rgb2gray(ReadImg);
   figure(2); imshow(GrayImg);title('Gray Image');
   %ROI selection manually
   height = 254   %189;
   width = 254   %229;
   [col1, row1] = ginput(1);
   row2 = row1 + height;
   col2 = col1 + width;
   CropedImg = ReadImg(row1:row2, col1:col2);
   figure(3); imshow(CropedImg); title('Region of Intrest');
   %Removal of spekel noise using Median filter
   medianFilteredIm2 = medfilt2(CropedImg,[4,4]);%using [4,4] window size getting better results
   %figure(3); imshow(medianFilteredIm1);title('output of median filter');
   figure(4); imshow(medianFilteredIm2);title('output of median filter');
   %Segmentation of Kidney part using imfreehand
   h = imfreehand();
   mask = createMask(h);
   medianFilteredIm2(~mask) = 0;
   figure(5); imshow(medianFilteredIm2);
   GrayThresh = 0.4; %graythresh(medianFilteredIm2);
   BinaryImg = im2bw(medianFilteredIm2,GrayThresh);
   figure(10); imshow(BinaryImg);title('Binary Image');
   filledBinaryImage = imfill(BinaryImg, 'holes');
   pixelsInROI = medianFilteredIm2(filledBinaryImage);
   numBlackPixels = sum(pixelsInROI == 0);
   numWhitePixels = sum(pixelsInROI == 255);
end

Following are images.

https://plus.google.com/photos/111799079095235591256/albums/5982916654912559041?hl=en_US

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Answer 1

Image Analyst 2014-2-21

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在 MATLAB Online 中打开

I don't understand the question you quoted (due to bad grammar), and don't understand yours either. But I think you want this:

numWhitePixels = sum(filledBinaryImage(:));
numBlackPixels = numel(filledBinaryImage) - numWhitePixels ;

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Image Analyst 2014-2-22

在 MATLAB Online 中打开

Well somehow you have a mask image (what you call ROI) - whether by hand drawing or some other method. This is one binary image. Then you have the other binary image where you threshold or something and it eats into the kidney.

numImagePixels = numel(mask);
numWhiteMaskPixels = sum(mask(:));
numBlackMaskPixels = numImagePixels  - numWhiteMaskPixels;
numWhiteKidneyPixels = sum(binaryImage(:));
numBlackKidneyPixels = numImagePixels  - numWhiteKidneyPixels;

So that's everything. You can get the black pixels just inside the kidney by subtracting.

blackKidneyPixels = numBlackKidneyPixels - numBlackMaskPixels;

Anything else you need can be gotten also from subtractions.

Renuka 2014-2-23

Thank you.I got pixel value. But in next step of image processing, I want to do classification for chronic kidney disease. Stages of CKD will be decided on black and white ratio .For that I have to separate kidney in 10 equidistant part by using 3x3 squre morphological operator.but wihout proper kidney image how will i do that? As shown in fig 1. I got output up to that level. but as shown in fig 2 and 3 I am not getting output.

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