How do I find the distance between two pixel co-ordinates

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I would like to find the distance between two pixel co-ordinates detected by the camera , whose screenshot has been attached below. the unit of co-ordinates is in pixel whereas I want to know the euclidean distance. By lukign at it, we can say that the distance is approx 4, but how I detect it from matlab? pdist doesn't work as the co-ords are in pixels.

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Image Analyst
Image Analyst 2014-5-20
See my attached spatial calibration demo.

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Emmanuel
Emmanuel 2014-5-20
awesome!

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