Segment extraction from an image in any direction

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Hi, i'm trying to extract a segment from an image in any direction. Suppose i have an 800X600 image (RGB) and i want to read a segment from point A(500,200) to point B(300,600). Is there any way i can get a column vector which contains the pixels values in that "diagonal"? i think it has to be some sort of interpolation between the nearest pixels. I don´t want to rotate the image, i need the values as they are. Regards.

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Image Analyst
Image Analyst 2014-7-4
Yes. You can use improfile()
x = [200, 600];
y = [500, 300];
values = improfile(grayImage, x, y);
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Image Analyst
Image Analyst 2014-7-5
That's using the same spacing as the original sampling. You can go higher for more "resolution" though it's just giving you more samples, not necessarily giving more information, or you can use a smaller number if you want a sampling less frequent than you have in the image. It's your choice. Do whatever you want considering the time to process and what you want to do with the final image you're building up.

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