Microscope image Matlab processing

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Hi everyone
i'm trying to analyze microscope images with Matlab.
  • What i would like:
Starting from an image, like img_di_partenza (attached, not the original resolution), i would like to filter it and highlight only the fibers.
  • What i managed to achieve
  1. - First thing, i do is bring the grayscale image with rgb2gray.
  2. - Increase the contrast with imadjust
  3. - Finally I have set 'manual threshold'.
I get more or less something like img_pos_elabora image.
NB. the two images uploaded are not the same, they are an example.
Problems
- The result has a high 'signal to noise ratio'.
- I have many images and therefore I would like to automate everything (I would like to skip the step of the manual threshold).
Can someone help me?
TY everyone

采纳的回答

Image Analyst
Image Analyst 2021-9-29
So you'll have to come up with an autothreshold algorithm. There are some in MATLAB such as imbinarize() and graythresh(). I'm also attaching my triangle threshold routine, which is very good for skewed histograms like you most commonly see. You can of course develop your own custom one if you want.
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