How can I enhance the gradient of the contour of the object in the image?

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sou
sou 2014-8-5
回答： Image Analyst 2014-8-30
My problem is to detect the contour of the cell and its nucleus. to detect the contour of the cell I did my job perfectly.but i can't proceed in detecting the nucleus of the cell.
my code is.
A=imread('D:\sou project\original\2.bmp');
s=strel('disk',1,0);%Structuring element
I3 = imadjust(A);
figure, imshow(I3);
D=im2bw(I3);%binary Image F=imerode(D,s);%Erode the image by structuring element figure,imshow(A);title('Original Image'); figure,imshow(D);title('Binary Image'); figure,imshow(D-F);title('Boundary extracted Image');
i have attached the cell image below...kindly help me

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Image Analyst
Image Analyst 2014-8-30
Most likely, you don't need to do that. You're basically saying "I need algorithm A to detect and measure B" but you didn't tell us what B is . You just said you need algorithm A and I don't think that is likely to be the algorithm you need.

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