See the Signal Processing Toolbox spectrogram function. It can produce the data you need from your .wav file, and one of the 3D plotting functions (consider mesh, surf, ribbon) can do the plots.
From the spectrogram documentation:
- [S,F,T] = spectrogram(...) returns a vector of frequencies, F, and a vector of times, T, at which the spectrogram is computed. F has length equal to the number of rows of S. T has length k (defined above) and the values in T correspond to the center of each segment.
