You can:
1) Create a dummy original image and store it in the handles structure of your GUI.
handles.DummyImage = OriginalImage;
guidata(hObject,handles);
2) In axes2, use something like:
imshow(handles.DummyImage); % example to display the image
%... do your processing.
to retrieve it in your axes 2 and do whatever you want with it. Youcan store anything you want in the handles structure of the GUI, so it's easy to pass variables between multiple callbacks.
