Help with Image SNR

2015 1 6
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更新时间：2015 1 6
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Can anyone suggest a good way to be able to
1: estimate the average signal of the bright spots 2: Estimage the DC background level 3: The std of the background
This is my typical image
I enclose the ascii data as unable to upload tiffs

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Jason
Jason 2015-1-6
1: I first of all try to get the background:
im_back=imopen(im,strel('disk',40)); %Get features with radius>40 (i.e.. background)
bk=mean(im_back(:));
%se=strel('disk',2);
%z4=imerode(z3,se);
z1=im-im_back; %Subtract Background
imshow(im_back);
2: I then do a median filter to remove any single pixels:
%Median Filter this background subtracted image
z2=medfilt2(z1,[2,2]);
z2( z2 < 0) = 0; %put any negative values to zero
3:Create Binary Image
%threshold on median processed, background subtracted image to create
%binary image, then fill holes
IM=z2;
%level1 = graythresh(double(z2))
mx=double(max(IM(:)))
me=mean(IM(:));
sd=std2(IM(:));
level=round(me+0.5*sd)
IM( IM < level) = 0;
IM( IM > level-1) = 1;
z3 = imfill(IM,'holes');
Im not sure Im going about this int he right way???

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Jason
Jason
2015-1-6

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Jason
Jason
2015-1-6

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