Valentino - just save the structure to handles in one callback so that the other callback has access to it. For example, if the following is the callback to choose and load the contents of a file
function pushbutton1_Callback(hObject, eventdata, handles)
% choose the file
% load the data into a structure myData
% add the structure to handles
handles.myData = myData;
% save the updated structure
guidata(hObject,handles);
Now in your other callback, you can access this structure directly as
function pushbutton2_Callback(hObject, eventdata, handles)
if isfield(handles,'myData')
% do something with myData
end
Try implementing the above and see what happens!
