Sean - image is the name of a built-in MATLAB function so it is not a good idea to name your variable image and so you should rename it to something else.
As for having your second callback be "aware" of the image that was chosen in the first callback, note the comment for the handles structure input
% handles structure with handles and user data (see GUIDATA)
You can update this structure with the image (user data) in the first callback so that any other callbacks that receive handles as an input will have access to it. Try something like
function pushbutton1_Callback(hObject, eventdata, handles)
[filename pathname] = uigetfile({'*.jpg';'*.bmp'},'File Selector');
handles.myImage = strcat(pathname, filename);
axes(handles.axes1);
imshow(handles.myImage)
set(handles.edit1,'string',filename);
set(handles.edit2,'string',image);
% save the updated handles object
guidata(hObject,handles);
In your second callback, just reference the loaded image as handles.myImage as
function pushbutton2_Callback(hObject, eventdata, handles)
if isfield(handles,'myImage')
% do stuff
end
Try the above and see what happens!
